| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Gretna Elite Academy GEA Gold 09B |
02077-0011B2U-0025 | Alex Vazquez |
| A2 | z-Infinite Sports World ISW Chaos |
03124-0011B2U-0002 | Manuel Lira |
| A3 | Nebraska FC NFC Blue 2009 |
03062-0011B2U-0127 | Justin Menichetti |
| A4 | Papillion Soccer Club PSC 2009B Maroon |
02016-0011B2U-0007 | Justin Fry |
| A5 | Omaha United Soccer Club Omaha United SC 2009B Black |
03133-0012B2U-0014 | Elias Ramirez |
| A6 | z-South Sioux City Soccer Club SSC Academy 10- B11U |
04056-0012B1U-0007 | Armand Garcia |