| Group | Club/Team Name | Team ID | Head Coach |
| A1 | United Association Football UAF 09G Blue |
03001-0011G2U-0012 | Jayme Arlen |
| A2 | Sporting Nebraska FC SOFC G BLACK 09 |
03036-001OSG2U-2081 | Mike Moran |
| A3 | Blair Soccer Club Blair FC Impact |
04019-0012G2U-1038 | Ben Huenink |
| A4 | Fremont Soccer Club FSC Fusion Gold 09/10 |
04054-0013G2U-0149 | Joshua Breeden |
| A5 | Bennington Soccer Club BENSC INFERNO (12G) |
03057-0011G2U-0021 | Trenton Vakiner |
| A6 | Council Bluffs Soccer Club CBSC 2009G |
0O001-0011G2U-0005 | Alex Trent |
| A7 | Gretna Elite Academy GEA Gold 09G |
02077-0011G2U-0024 | Chris Mellott |